N-Acetyl Epitalon 10mg

Synonyms / Designations: N-Acetyl Epitalon; N-Acetyl Epithalon; Ac-Epitalon; N-acetylated AEDG (Ac-Ala-Glu-Asp-Gly-OH)
Amino Acid Sequence: Ac-Ala-Glu-Asp-Gly-OH (N-terminally acetylated AEDG tetrapeptide)
CAS Number: N/A
Molecular Formula: C16H24N4O10
Molecular Weight: 432.4 g/mol
Purity: ≥ 99 % (HPLC)
Appearance: White to off-white lyophilised powder
Pack Size: 10 mg (total)
Storage: Desiccated, protected from light, stored at –20 °C or below
Solubility: Soluble in water and buffered aqueous solutions

Description & Mechanism

N-Acetyl Epitalon is an N-terminally acetylated analogue of the synthetic tetrapeptide Epitalon (AEDG), used as a research reagent to study the parent peptide’s reported activities in experimental systems. In cell and tissue models, Epitalon-family peptides have been studied as putative modulators of telomerase (TERT) expression and as short peptides proposed to interact with DNA/chromatin to influence gene-expression patterns.

N-terminal acetylation is a common chemical modification intended to reduce aminopeptidase cleavage and improve in-vitro stability and handling relative to the unmodified peptide. As a laboratory probe the molecule is used to interrogate proposed peptide–gene-regulation effects, antioxidant/cytoprotective endpoints and senescence-related markers in cell culture; described effects are mechanistic and in-vitro in nature.

Applications in Research

• In-vitro studies of telomerase (TERT) expression and replicative senescence in cultured cells
• Cellular models probing short-peptide modulation of gene expression / chromatin interaction
• Antioxidant and cytoprotective endpoint assays in cell culture
• Comparative stability studies of acetylated vs native tetrapeptides (peptide chemistry research)

Specifications Summary

Handling, Reconstitution & Stability

• Weigh under dry conditions; peptide is hygroscopic
• Reconstitute in sterile water or appropriate buffered aqueous solution depending on assay requirements
• Avoid vigorous agitation during dissolution
• Filter sterilize if required (e.g. 0.22 µm) prior to use
• Aliquot and store reconstituted solutions at –20 °C or below to minimise degradation
• Avoid repeated freeze–thaw cycles

Precautions & Notes

• Confirm the exact entity supplied (free-acid Ac-AEDG-OH vs amidated Ac-AEDG-NH2) against the batch CoA; molecular weights differ
• Experimental behaviour is dependent on concentration, model system and exposure conditions
• Prolonged exposure to light, elevated temperature or aqueous environments may reduce stability
• Intended strictly for laboratory research use; not for human or veterinary application